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pMSCV-ALDH1A3
(Plasmid #260551)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 260551 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pMSCVpuro
  • Backbone manufacturer
    Addgene
  • Backbone size w/o insert (bp) 6300
  • Total vector size (bp) 7838
  • Modifications to backbone
    Included ALDH1A3 coding sequence
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ALDH1A3 coding sequence
  • Insert Size (bp)
    1538
  • GenBank ID
    NM_000693
  • Entrez Gene
    ALDH1A3 (a.k.a. ALDH1A6, ALDH6, MCOP8, RALDH3)
  • Promoter LTR

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer 5'-CCCTTGAACCTCCTCGTTCGACC-3'
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMSCV-ALDH1A3 was a gift from Paola Marcato (Addgene plasmid # 260551 ; http://n2t.net/addgene:260551 ; RRID:Addgene_260551)

  • For your References section:

    Aldehyde dehydrogenase 1A3 influences breast cancer progression via differential retinoic acid signaling. Marcato P, Dean CA, Liu RZ, Coyle KM, Bydoun M, Wallace M, Clements D, Turner C, Mathenge EG, Gujar SA, Giacomantonio CA, Mackey JR, Godbout R, Lee PW. Mol Oncol. 2015 Jan;9(1):17-31. doi: 10.1016/j.molonc.2014.07.010. Epub 2014 Jul 24. 10.1016/j.molonc.2014.07.010 PubMed 25106087
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