pMSCV-ALDH1A1
(Plasmid #260552)

• Purpose: Expressing human ALDH1A1 using the pMSCVpuro retroviral vector is a standard, robust method for generating stable overexpressing cell lines
• Depositing Lab: Paola Marcato
• Depositing Organization: Dalhousie University
  Located in Canada
• Publication: Marcato et al Mol Oncol. 2015 Jan;9(1):17-31. doi: 10.1016/j.molonc.2014.07.010. Epub 2014 Jul 24.

Backbone

• Vector backbone: pMSCVpuro
• Backbone size w/o insert (bp): 6300
• Total vector size (bp): 7800
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: ALDH1A1 coding sequence
• Species: H. sapiens (human)
• Insert Size (bp): 1503
• GenBank ID: NM_000689
• Entrez Gene: ALDH1A1 (a.k.a. ALDC, ALDH-E1, ALDH1, ALDH11, HEL-9, HEL-S-53e, HEL12, PUMB1, RALDH1)
• Promoter: LTR

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: XhoI (unknown if destroyed)
• 5′ sequencing primer: 5'-CCCTTGAACCTCCTCGTTCGACC-3'

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry