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pET21a(+)-Thio6-His6-TEV-TARDBP(Δ1-273)
(Plasmid #260633)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 260633 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pET21a(+)
  • Backbone manufacturer
    EMD Biosciences
  • Backbone size w/o insert (bp) 5443
  • Total vector size (bp) 5847
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Transactive response DNA binding protein 43
  • Alt name
    TDP-43
  • Alt name
    TARDBP
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    480
  • Mutation
    deleted amino acids 1-273
  • Entrez Gene
    TARDBP (a.k.a. ALS10, TDP-43)
  • Tag / Fusion Protein
    • thio6 expression sequence, hexahistidine, TEV protease recognition sequence (N terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET21a(+)-Thio6-His6-TEV-TARDBP(Δ1-273) was a gift from Jennifer Lee (Addgene plasmid # 260633 ; http://n2t.net/addgene:260633 ; RRID:Addgene_260633)

  • For your References section:

    Site-Specific Raman Probes Reveal Droplet Aging and Residue-Level Fibril Polymorphism in TDP-43(CTD). Watson MD, Lee JC. J Am Chem Soc. 2026 Jun 17;148(23):23494-23498. doi: 10.1021/jacs.6c07727. Epub 2026 Jun 2. 10.1021/jacs.6c07727 PubMed 42227825
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