TrmA design 2
(Plasmid
#260655)
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PurposeBacterial expression of a computationally designed TrmA based on Pseuodomonas thermotolerans TrmA
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Depositing Lab
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Depositing OrganizationUniversity of Washington
Located in the United States of America -
Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 260655 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepNIC28-Bsa4
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameTrmA
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SpeciesSynthetic
- Promoter T7
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Tag
/ Fusion Protein
- 6xHis (N terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
TrmA design 2 was a gift from Stan Fields (Addgene plasmid # 260655 ; http://n2t.net/addgene:260655 ; RRID:Addgene_260655)
