pDosa2
(Plasmid
#260656)
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Purpose(Empty Backbone) Updated cloning vector to express a fusion of a variant protein and a tRNA modifying enzyme TrmA from Pseudomonas thermotolerans, to covalently link an RNA barcode
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 260656 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepET-LIC-cloning vector (2A-T)
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameNone
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Protein of interest to be cloned into BsaI site. Barcodes to be inserted into Esp3I site. TrmA (E354Q). Barcodes flanked by Nextera primer sequences.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDosa2 was a gift from Stan Fields (Addgene plasmid # 260656 ; http://n2t.net/addgene:260656 ; RRID:Addgene_260656)
