WT Ubc9-TrmA
(Plasmid #260663)

• Purpose: Bacterial expression of yeast Ubc9 fused with Pseudomonas thermotolerans TrmA tRNA crosslinking with RNA barcode
• Depositing Lab: Stan Fields
• Depositing Organization: University of Washington
  Located in the United States of America
• Publication: Fields Lab Additional pDosa2 Plasmids (unpublished)

Backbone

• Vector backbone: pET-LIC-cloning vector (2A-T)
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Ubc9
• Species: S. cerevisiae (budding yeast)
• Promoter: T7
• Tag / Fusion Protein:
  • TrmA (C terminal on insert)

Cloning Information

• Cloning method: Golden Gate

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry