6X-His-FLAG-pDosa2
(Plasmid #260665)

• Purpose: Bacterial expression of Pseudomonas thermotolerans TrmA for tRNA crosslinking with RNA barcode
• Depositing Lab: Stan Fields
• Depositing Organization: University of Washington
  Located in the United States of America
• Publication: Fields Lab pDosa2 Plasmids (unpublished)

Backbone

• Vector backbone: pDosa2
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: TrmA
• Species: Pseudomonas thermotolerans
• Promoter: T7
• Tag / Fusion Protein:
  • 6xHis-FLAG (N terminal on insert)

Cloning Information

• Cloning method: Golden Gate

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry