CAII H107Y-pDosa2
(Plasmid
#260671)
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PurposeBacterial expression of human carbonic anhydrase 2 (CAII) mutant fused to Pseudomonas thermotolerans TrmA for tRNA crosslinking with RNA barcode
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Depositing Lab
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Depositing OrganizationUniversity of Washington
Located in the United States of America -
Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 260671 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepDosa2
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameCAII
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SpeciesH. sapiens (human)
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MutationH107Y
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Entrez GeneCA2 (a.k.a. CA-II, CAC, CAII, Car2, HEL-76, HEL-S-282)
- Promoter T7
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Tag
/ Fusion Protein
- TrmA (C terminal on backbone)
Cloning Information
- Cloning method Golden Gate
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
CAII H107Y-pDosa2 was a gift from Stan Fields (Addgene plasmid # 260671 ; http://n2t.net/addgene:260671 ; RRID:Addgene_260671)
