Anti-Parvalbumin [L114/3R-Rb] LC
(Plasmid
#260707)
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PurposeMammalian expression plasmid of Anti-Parvalbumin (Rat) chimeric rabbit recombinant antibody light chain with fused mouse variable domains; use with Addgene #260706 to generate full length antibody.
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Depositing Labs
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 260707 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepcDNA3.1
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Vector typeMammalian Expression, Affinity Reagent/ Antibody
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameAnti-Parvalbumin (Rattus norvegicus) recombinant mouse monoclonal antibody light chain.
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SpeciesPvalb Chimera: Mus musculus (mouse)/Oryctolagus cuniculus (rabbit)
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Insert Size (bp)693
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MutationNone
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Entrez GenePvalb (a.k.a. PALB1, Pva)
- Promoter CMV
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer Unknown
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Must be used with Anti-Parvalbumin [L114/3R-Rb] HC plasmid (Addgene #260706) to generate full length antibody: https://www.addgene.org/260706/.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Anti-Parvalbumin [L114/3R-Rb] LC was a gift from Karl Murray & Zhuhao Wu (Addgene plasmid # 260707 ; http://n2t.net/addgene:260707 ; RRID:Addgene_260707) -
For your References section:
High-volume hybridoma sequencing on the NeuroMabSeq platform enables efficient generation of recombinant monoclonal antibodies and scFvs for neuroscience research. Mitchell KG, Gong B, Hunter SS, Burkart-Waco D, Gavira-O'Neill CE, Templeton KM, Goethel ME, Bzymek M, MacNiven LM, Murray KD, Settles ML, Froenicke L, Trimmer JS. Sci Rep. 2023 Sep 27;13(1):16200. doi: 10.1038/s41598-023-43233-4. 10.1038/s41598-023-43233-4 PubMed 37758930