pCW57.1 Flag-FOXM1c DBD MT
(Plasmid #260711)

• Purpose: Doxycycline inducible lentiviral vector for FOXM1c with DNA binding domain mutation at R286A, H287A, and S290A
• Depositing Lab: Adam Karpf
• Depositing Organization: University of Nebraska Medical Center
  Located in the United States of America
• Publication: Barger et al Cancers (Basel). 2019 Feb 21;11(2). pii: cancers11020251. doi: 10.3390/cancers11020251.

Backbone

• Vector backbone: pCW57.1
• Backbone manufacturer: Broad Insitute
• Vector type: Mammalian Expression, Lentiviral ; Doxycycline inducible
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: FOXM1c
• Alt name: Forkhead Box M1c
• Alt name: Forkhead Box M1
• Alt name: FOXM1
• Species: H. sapiens (human)
• Insert Size (bp): 2331
• Mutation: R286A, H267A, and S290A
• GenBank ID: NM_021953
• Entrez Gene: FOXM1 (a.k.a. FKHL16, FOXM1A, FOXM1B, FOXM1C, HFH-11, HFH11, HNF-3, INS-1, MPHOSPH2, MPP-2, MPP2, PIG29, TRIDENT)
• Tag / Fusion Protein:
  • Flag (N terminal on insert)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry