pL4440-CENURA
(Plasmid
#260751)
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Purpose(Empty Backbone) Contains opposing T7 promoters for double-stranded RNA expression in HT115. Contains a yeast centromere and Ura3 gene for propagation, recombination, and selection in yeast.
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Depositing Lab
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Depositing OrganizationConverse University
Located in United States of America -
Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 260751 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepL4440
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Backbone manufacturerAndrew Fire (Plasmid #1654)
- Backbone size (bp) 4628
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Modifications to backboneInsertion of yeast centromere and Ura3 into PciI site
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Vector typeRNAi
- Promoter T7
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Selectable markersURA3
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Cloning Information
- Cloning method Other
- 5′ sequencing primer M13F
- (Common Sequencing Primers)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pL4440-CENURA was a gift from Edward Griffin (Addgene plasmid # 260751 ; http://n2t.net/addgene:260751 ; RRID:Addgene_260751)
Map uploaded by the depositor.
