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pLVX-TetOne-Puro-mitoTPNOX-FLAG
(Plasmid #260846)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 260846 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pLVX-TetOne-Puro
  • Backbone manufacturer
    Clontech Laboratories
  • Backbone size w/o insert (bp) 9172
  • Total vector size (bp) 10648
  • Vector type
    Mammalian Expression, Lentiviral, Synthetic Biology
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    mitoTPNOX
  • Alt name
    NADPH specific mutant of mitoLbNOX
  • Species
    H.sapiens codon optimized bacterial gene
  • Insert Size (bp)
    1476
  • Tag / Fusion Protein
    • FLAG (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site AgeI (not destroyed)
  • 5′ sequencing primer AGTAAACTTCAATTCCACAACACTTTTGTC
  • 3′ sequencing primer CTCTTACCCGTCATTGGCTGT
  • (Common Sequencing Primers)

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLVX-TetOne-Puro-mitoTPNOX-FLAG was a gift from Valentin Cracan (Addgene plasmid # 260846 ; http://n2t.net/addgene:260846 ; RRID:Addgene_260846)

  • For your References section:

    A genetically encoded tool to increase cellular NADH/NAD(+) ratio in living cells. Pan X, Heacock ML, Abdulaziz EN, Violante S, Zuckerman AL, Shrestha N, Yao C, Goodman RP, Cross JR, Cracan V. Nat Chem Biol. 2023 Oct 26. doi: 10.1038/s41589-023-01460-w. 10.1038/s41589-023-01460-w PubMed 37884806
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