MAGE-L2-EGFP-pIRES
(Plasmid
#260887)
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PurposeExpresses human full length MAGE-L2 with a N-terminal EGFP tag in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 260887 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepIRES puro
- Total vector size (bp) 7496
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Vector typeMammalian Expression
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namehuman MAGE-L2
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SpeciesH. sapiens (human)
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Insert Size (bp)1503
- Promoter CMV
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Tag
/ Fusion Protein
- EGFP (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site FseI (not destroyed)
- 3′ cloning site AscI (not destroyed)
- 5′ sequencing primer EGFP-N
- (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byKlementina Fon Tacer, DVM, PhD CPRIT Scholar Assistant Professor, Cancer Biology and Reproductive Biology Director, Texas Center for Comparative Cancer Research
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.64898/2026.04.24.720667 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
MAGE-L2-EGFP-pIRES was a gift from Irina Bezsonova (Addgene plasmid # 260887 ; http://n2t.net/addgene:260887 ; RRID:Addgene_260887)