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Purpose(Empty Backbone) SGC Empty backbone for bacterial expression
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 26101 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * | |
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Backbone
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Vector backbonepET28GST-LIC
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Backbone manufacturerStructural Genomics Consortium
- Backbone size (bp) 7998
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameNone
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Tags
/ Fusion Proteins
- GST (N terminal on backbone)
- 6XHis (N terminal on backbone)
- Thrombin CLeavage Site (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer T7, pGEX 5' (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET28GST-LIC was a gift from Cheryl Arrowsmith (Addgene plasmid # 26101 ; http://n2t.net/addgene:26101 ; RRID:Addgene_26101)
Map uploaded by the depositor.
