pMS2-CAGGS-EGFP
(Plasmid
#261075)
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PurposeTemplate plasmid to insert homology arms via Golden Gate assembly for endogenous knock-in of MS2 stem loops via HDR. Contains loxP-flanked selection marker EGFP that is removable by Cre recombination.
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Depositing Lab
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Depositing OrganizationUniversity of Zurich
Located in Switzerland -
Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 261075 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepBluescriptKS
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert name24 x MS2v6-3nt-linker stem loop array
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Insert Size (bp)624
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer T7
- (Common Sequencing Primers)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMS2-CAGGS-EGFP was a gift from Franka Voigt (Addgene plasmid # 261075 ; http://n2t.net/addgene:261075 ; RRID:Addgene_261075)
Map uploaded by the depositor.
