pLV-TetO-hNGN2-hygro
(Plasmid
#261079)
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PurposeVector for lentiviral insertion of dox-inducible (TetO) human NGN2, coupled to hygromycin resistance gene.
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Depositing Lab
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Depositing OrganizationUniversity of Zurich
Located in Switzerland -
Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 261079 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepTet-O-Ngn2-puro (#52047)
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Backbone manufacturerMarius Wernig
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Vector typeLentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert namehNGN2-P2A-hygro
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SpeciesH. sapiens (human)
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Entrez GeneNEUROG2 (a.k.a. Atoh4, Math4A, NGN2, bHLHa8, ngn-2)
- Promoter tetO
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer n/a
- (Common Sequencing Primers)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLV-TetO-hNGN2-hygro was a gift from Franka Voigt (Addgene plasmid # 261079 ; http://n2t.net/addgene:261079 ; RRID:Addgene_261079)