Skip to main content
Addgene
Open Menu

pGL3-(CHM)-c.-1093_-31
(Plasmid #261177)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 261177 Standard format: Plasmid sent in bacteria as agar stab 1 $94
Add to Cart

Backbone

  • Vector backbone
    pGL3
  • Backbone size w/o insert (bp) 4818
  • Total vector size (bp) 5841
  • Vector type
    Mammalian Expression, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CHM upstream region
  • Alt name
    REP1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1073
  • Entrez Gene
    CHM (a.k.a. DXS540, GGTA, HSD-32, REP-1, TCD)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SacI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer CAA AAT AGG CTG TCC CCA GTG
  • (Common Sequencing Primers)

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGL3-(CHM)-c.-1093_-31 was a gift from Ian MacDonald (Addgene plasmid # 261177 ; http://n2t.net/addgene:261177 ; RRID:Addgene_261177)

  • For your References section:

    Single-base substitutions in the CHM promoter as a cause of choroideremia. Radziwon A, Arno G, K Wheaton D, McDonagh EM, Baple EL, Webb-Jones K, G Birch D, Webster AR, MacDonald IM. Hum Mutat. 2017 Jun;38(6):704-715. doi: 10.1002/humu.23212. Epub 2017 Mar 24. 10.1002/humu.23212 PubMed 28271586
Related items: