MYC-5pTAD-96XTetO
(Plasmid
#261185)
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PurposeDonor plasmid for insertion of 96XTetO + Zeocin resistance gene at 5' boundary of human MYC TAD
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Depositing Lab
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Depositing OrganizationNational Cancer Institute (NCI)
Located in the United States of America -
Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 261185 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backboneDonor DNA template
- Total vector size (bp) 10060
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Vector typeMammalian Expression, CRISPR
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Selectable markersZeocin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert 1
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Gene/Insert name96XTetO
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SpeciesSynthetic
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Insert Size (bp)3299
Gene/Insert 2
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Gene/Insert nameBleoR
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Alt nameZeocin resistance gene
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SpeciesSynthetic
Resource Information
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A portion of this plasmid was derived from a plasmid made byMade by Genome Modification Core, Frederick National Laboratory for Cancer Research, USA
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
MYC-5pTAD-96XTetO was a gift from Tom Misteli (Addgene plasmid # 261185 ; http://n2t.net/addgene:261185 ; RRID:Addgene_261185)