ERRFI1-3pTAD-130XmTetO
(Plasmid
#261186)
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PurposeDonor plasmid for insertion of 130XmutTetO + Neomycin resistance gene at 3' boundary of human ERRFI1 TAD
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Depositing Lab
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Depositing OrganizationNational Cancer Institute (NCI)
Located in the United States of America -
Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 261186 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backboneDonor DNA template
- Total vector size (bp) 10760
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Vector typeMammalian Expression, CRISPR
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert 1
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Gene/Insert name130XmTetO
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SpeciesSynthetic
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Insert Size (bp)4000
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MutationMutated half site of TetO sequences
Gene/Insert 2
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Gene/Insert nameNeoR
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Alt nameNeomycin resistance gene
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SpeciesSynthetic
Resource Information
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A portion of this plasmid was derived from a plasmid made byMade partially by Genome Modification Core, Frederick National Laboratory for Cancer Research, USA
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Addgene provided sequence with 133XmTetO repeats is the full, correct sequence of the plasmid. Depositor’s sequence with 103XmTetO repeats is provided as a reference for relevant plasmid features.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
ERRFI1-3pTAD-130XmTetO was a gift from Tom Misteli (Addgene plasmid # 261186 ; http://n2t.net/addgene:261186 ; RRID:Addgene_261186)