pHAGE_TetR-mTetR-3XmCh-CRY2
(Plasmid
#261191)
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PurposeLentiviral vector for stable expression of TetR-mutTetR-3XmCherry-CRY2 fusion protein in mammalian cells
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Depositing Lab
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Depositing OrganizationNational Cancer Institute (NCI)
Located in the United States of America -
Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 261191 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepHAGE-DEST
- Total vector size (bp) 11787
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Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert 1
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Gene/Insert nameCRY2hiclu
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Alt nameCRY2PHR E490R/DLDN
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SpeciesA. thaliana (mustard weed)
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Insert Size (bp)1521
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GenBank ID839529
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Tag
/ Fusion Protein
- 3XmCherry (N terminal on insert)
Gene/Insert 2
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Gene/Insert nameTetR-mTetR
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Alt nameTet repressor
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SpeciesSynthetic
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Insert Size (bp)1377
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MutationE37A/P39K for mTetR
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Tag
/ Fusion Protein
- 3XmCherry (C terminal on insert)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pHAGE_TetR-mTetR-3XmCh-CRY2 was a gift from Tom Misteli (Addgene plasmid # 261191 ; http://n2t.net/addgene:261191 ; RRID:Addgene_261191)