pAM049_pMi[3xP3::mCherry-SV40,ivory1.5kb_prom::mBaoJin-P10UTR]
(Plasmid
#261229)
-
PurposeMinos transgenesis construct for Lepidoptera expressing a cytoplasmic mBaoJin fluorophore in developing wing scales
-
Depositing Lab
-
Depositing OrganizationGeorge Washington University
Located in United States of America -
Publication
-
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 261229 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
-
Vector backbonepBluescript, pMi(3xP3-EGFP)
-
Vector typeInsect Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert name3xP3::mCherry-SV40,ivory1.5kb_prom::mBaoJin-P10UTR
-
SpeciesVanessa cardui
-
Insert Size (bp)4889
- Promoter 3xP3 ; ivory1.5kb_prom
Cloning Information
- Cloning method Gibson Cloning
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Ivory1.5kb_prom::mBaoJin-P10UTR: Synthetic gene for labeling the cytoplasm of developing wing scales, using the Vanessa cardui promoter of the ivory gene
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pAM049_pMi[3xP3::mCherry-SV40,ivory1.5kb_prom::mBaoJin-P10UTR] was a gift from Arnaud Martin (Addgene plasmid # 261229 ; http://n2t.net/addgene:261229 ; RRID:Addgene_261229)