pAM057_pMi[ivory1.5kb_prom::mScarlet3-KRas6R-SV40, Pi_FibL:mBaoJin-P10UTR]
(Plasmid
#261232)
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PurposeMinos transgenesis construct for Lepidoptera expressing a red-fluorescent membrane tag in developing wing scales
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Depositing Lab
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Depositing OrganizationGeorge Washington University
Located in United States of America -
Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 261232 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepBluescript, pMi(3xP3-EGFP)
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Vector typeInsect Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameivory1.5kb_prom::mScarlet3-KRas6R-SV40, Pi_FibL:mBaoJin-P10UTR
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SpeciesPlodia interpunctella, Vanessa cardui
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Insert Size (bp)5704
- Promoter ivory1.5kb_prom, Pi_FibL
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer Unspecified
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Ivory1.5kb_prom::mScarlet3-KRas6R-SV40: Expression of a membrane-tagged mScarlet3 red fluorophore in developing wing scales of lepidopteran insects.
Pi_FibL:mBaoJin-P10UTR: Synthetic gene fragment consisting of the Plodia interpunctella FibL promoter (drives expression in the Posterior Silk Gland), and expressing the mBaoJin fluorescent marker.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAM057_pMi[ivory1.5kb_prom::mScarlet3-KRas6R-SV40, Pi_FibL:mBaoJin-P10UTR] was a gift from Arnaud Martin (Addgene plasmid # 261232 ; http://n2t.net/addgene:261232 ; RRID:Addgene_261232)