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pCAGGS-SHH
(Plasmid #261260)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 261260 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pCAG
  • Total vector size (bp) 4900
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Sonic hedgehog
  • Alt name
    SHH
  • Species
    G. gallus (chicken)
  • Insert Size (bp)
    1275
  • Entrez Gene
    SHH (a.k.a. ShhNC)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (not destroyed)
  • 3′ cloning site Bsu36I (not destroyed)
  • 5′ sequencing primer pCAGGS-5
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAGGS-SHH was a gift from Cliff Tabin (Addgene plasmid # 261260 ; http://n2t.net/addgene:261260 ; RRID:Addgene_261260)

  • For your References section:

    Convergent flow-mediated mesenchymal force drives embryonic foregut constriction and splitting. Yan R, Hoffmann LA, Oikonomou P, Li D, Lee C, Gill HK, Mongera A, Nerurkar NL, Mahadevan L, Tabin CJ. Nat Commun. 2025 Nov 27;16(1):10643. doi: 10.1038/s41467-025-65644-9. 10.1038/s41467-025-65644-9 PubMed 41309607
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