pAdaptamer-AAV_V9-18_Tet_FGF21
(Plasmid
#261300)
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PurposeTetracycline adaptamer controlled hFGF21, AAV backbone
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Depositing Lab
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Depositing OrganizationUniversity of California, San Diego (UCSD)
Located in the United States of America -
Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 261300 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepZac
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Vector typeMammalian Expression, AAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameV9-18_hFGF21
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SpeciesH. sapiens (human)
- Promoter CMV
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer CGCAAATGGGCGGTAGGCGTG
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAdaptamer-AAV_V9-18_Tet_FGF21 was a gift from Prashant Mali (Addgene plasmid # 261300 ; http://n2t.net/addgene:261300 ; RRID:Addgene_261300) -
For your References section:
Regulatable in vivo gene expression via adaptamers. Bryant J, Herron L, Doctor Y, McRae F, Wang J, Hirsch T, Portell A, Kumar S, Mali P. Molecular Cell 10.1016/j.molcel.2026.09.037