pCORE_04__pUC_SpecR_LacZ
(Plasmid
#261304)
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Purpose(Empty Backbone) This plasmid can fuction as Moclo Level 1 (BsaI) or Level 2 (BpiI) acceptors and as starting backbones for developing vectors for other hosts through AarI-mediated Golden Gate assembly.
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Depositing Lab
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Depositing OrganizationMRC Laboratory of Medical Sciences
Located in United Kingdom of Great Britain and Northern Ireland -
Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 261304 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backboneUnspecified
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Vector typeOther
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
BsaI for MoClo Level 1, BpiI for MoClo Level 2, sites destroyed during cloning
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCORE_04__pUC_SpecR_LacZ was a gift from Karen Sarkisyan (Addgene plasmid # 261304 ; http://n2t.net/addgene:261304 ; RRID:Addgene_261304)
