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pUAST-Pallidin-Flag
(Plasmid #261357)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 261357 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pUAST
  • Backbone manufacturer
    flybase
  • Backbone size w/o insert (bp) 9035
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Pallidin
  • Alt name
    Snapin
  • Species
    D. melanogaster (fly)
  • Insert Size (bp)
    504
  • Entrez Gene
    pallidin (a.k.a. Dmel_CG14133, CG14133, Dmel\CG14133)
  • Tag / Fusion Protein
    • Flag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    pOT2-Pallidin

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

infusion cloning + restriction enzyme

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUAST-Pallidin-Flag was a gift from Claire Benetollo (Addgene plasmid # 261357 ; http://n2t.net/addgene:261357 ; RRID:Addgene_261357)
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