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HsRPE65_Y338F
(Plasmid #261372)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 261372 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone size w/o insert (bp) 5375
  • Total vector size (bp) 6974
  • Vector type
    Mammalian Expression, Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Human RPE65 Y338F
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1599
  • Entrez Gene
    RPE65 (a.k.a. BCO3, LCA2, RP20, mRPE65, p63, rd12, sRPE65)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Noti (unknown if destroyed)
  • 3′ cloning site Hindiii (unknown if destroyed)
  • 5′ sequencing primer AAT ACG ACT CAC TAT AG
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HsRPE65_Y338F was a gift from Martin Horvath (Addgene plasmid # 261372 ; http://n2t.net/addgene:261372 ; RRID:Addgene_261372)
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