GgRPE65_Y338Q
(Plasmid
#261373)
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PurposeBacterial and Mammalian expression of chicken RPE65 with Tyr338 with uncharged Gln338.
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Depositing Lab
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Depositing OrganizationUniversity of Utah
Located in United States of America -
Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 261373 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepcDNA3.1
- Backbone size w/o insert (bp) 5375
- Total vector size (bp) 6974
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Vector typeMammalian Expression, Bacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameChicken RPE65 Y338Q
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SpeciesG. gallus (chicken)
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Entrez GeneRPE65
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Noti (unknown if destroyed)
- 3′ cloning site Hindiii (unknown if destroyed)
- 5′ sequencing primer AAT ACG ACT CAC TAT AG
- (Common Sequencing Primers)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
GgRPE65_Y338Q was a gift from Martin Horvath (Addgene plasmid # 261373 ; http://n2t.net/addgene:261373 ; RRID:Addgene_261373)