pJYW613
(Plasmid
#261395)
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PurposeExpressing sulfite reductase under E. coli CysB promoter
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Depositing Lab
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Depositing OrganizationFlorida State University, Tallahassee
Located in United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 261395 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepBR322
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namecysJIG
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Alt nameSiRFP
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Alt nameSiRHP
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Alt nameCysG
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pJYW613 was a gift from M. Elizabeth Stroupe (Addgene plasmid # 261395 ; http://n2t.net/addgene:261395 ; RRID:Addgene_261395) -
For your References section:
High-level expression of Escherichia coli NADPH-sulfite reductase: requirement for a cloned cysG plasmid to overcome limiting siroheme cofactor. Wu JY, Siegel LM, Kredich NM. J Bacteriol. 1991 Jan;173(1):325-33. doi: 10.1128/jb.173.1.325-333.1991. 10.1128/jb.173.1.325-333.1991 PubMed 1987123