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pBAD-cysJIG(his-J)
(Plasmid #261396)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 261396 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pBAD-his/mycA
  • Backbone size w/o insert (bp) 4020
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    cysJIG
  • Alt name
    SiRFP
  • Alt name
    SiRHP
  • Alt name
    CysG
  • Insert Size (bp)
    4958
  • Promoter araC
  • Tag / Fusion Protein
    • 6xhis on SiRFP (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer ATG CCA TAG CAT TTT TAT CC
  • 3′ sequencing primer GAT TTA ATC TGT ATC AGG
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBAD-cysJIG(his-J) was a gift from M. Elizabeth Stroupe (Addgene plasmid # 261396 ; http://n2t.net/addgene:261396 ; RRID:Addgene_261396)

  • For your References section:

    The N-terminal Domain of Escherichia coli Assimilatory NADPH-Sulfite Reductase Hemoprotein Is an Oligomerization Domain That Mediates Holoenzyme Assembly. Askenasy I, Pennington JM, Tao Y, Marshall AG, Young NL, Shang W, Stroupe ME. J Biol Chem. 2015 Jul 31;290(31):19319-33. doi: 10.1074/jbc.M115.662379. Epub 2015 Jun 18. 10.1074/jbc.M115.662379 PubMed 26088143
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