pBADcysIG
(Plasmid
#261398)
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PurposeExpresses untagged sulfite reductase hemoprotein (cysI) with siroheme synthase (cysG)
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Depositing Lab
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Depositing OrganizationFlorida State University, Tallahassee
Located in United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 261398 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepBAD-his/mycA
- Backbone size w/o insert (bp) 4020
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namecysIG
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Alt nameSiRHP
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Alt nameCysG
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Insert Size (bp)3135
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Tag
/ Fusion Protein
- None
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBADcysIG was a gift from M. Elizabeth Stroupe (Addgene plasmid # 261398 ; http://n2t.net/addgene:261398 ; RRID:Addgene_261398) -
For your References section:
The N-terminal Domain of Escherichia coli Assimilatory NADPH-Sulfite Reductase Hemoprotein Is an Oligomerization Domain That Mediates Holoenzyme Assembly. Askenasy I, Pennington JM, Tao Y, Marshall AG, Young NL, Shang W, Stroupe ME. J Biol Chem. 2015 Jul 31;290(31):19319-33. doi: 10.1074/jbc.M115.662379. Epub 2015 Jun 18. 10.1074/jbc.M115.662379 PubMed 26088143