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pEF1a-H3.3-HaloTag-FRT
(Plasmid #261404)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 261404 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pcDNA5/FRT
  • Modifications to backbone
    Replace CMV promoter sequence with EF1a promoter.
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Homo sapiens H3.3 histone
  • Species
    H. sapiens (human)
  • GenBank ID
    AM393192
  • Entrez Gene
    H3-3B (a.k.a. BRYLIB2, H3.3B, H3F3B)
  • Promoter EF1a
  • Tag / Fusion Protein
    • HaloTag (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2025.08.27.672592 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEF1a-H3.3-HaloTag-FRT was a gift from Kazuhiro Maeshima (Addgene plasmid # 261404 ; http://n2t.net/addgene:261404 ; RRID:Addgene_261404)

  • For your References section:

    Cohesin prevents local mixing of condensed euchromatic domains in living human cells. Shimazoe MA, Iida S, Minami K, Higashi K, Tamura S, Kobayashi Y, Fujishiro S, Xiong L, Nakazato K, Ashwin SS, Nishiyama T, Nagata Y, Kanemaki MT, Kawaguchi A, Ohkawa Y, Schermelleh L, Toyoda A, Xie L, Kurokawa K, Ochiai H, Sasai M, Maeshima K. Nat Genet. 2026 Sep;58(9):2335-2349. doi: 10.1038/s41588-026-02736-2. Epub 2026 Sep 8. 10.1038/s41588-026-02736-2 PubMed 42711428
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