Skip to main content
Addgene
Open Menu

pPB-EF1a-H3.3-HaloTag
(Plasmid #261405)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 261405 Standard format: Plasmid sent in bacteria as agar stab 1 $94
Add to Cart
DNA 261405-D50 50 μg of DNA in Tris buffer $495
Add to Cart
DNA 261405-D100 100 μg of DNA in Tris buffer $585
Add to Cart

Backbone

  • Vector backbone
    pPB-PGKneopA
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Homo sapiens H3.3 histone
  • Species
    H. sapiens (human)
  • GenBank ID
    AM393192
  • Entrez Gene
    H3-3B (a.k.a. BRYLIB2, H3.3B, H3F3B)
  • Promoter EF1a
  • Tag / Fusion Protein
    • HaloTag (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2025.08.27.672592 for bioRxiv preprint.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPB-EF1a-H3.3-HaloTag was a gift from Kazuhiro Maeshima (Addgene plasmid # 261405 ; http://n2t.net/addgene:261405 ; RRID:Addgene_261405)

  • For your References section:

    Cohesin prevents local mixing of condensed euchromatic domains in living human cells. Shimazoe MA, Iida S, Minami K, Higashi K, Tamura S, Kobayashi Y, Fujishiro S, Xiong L, Nakazato K, Ashwin SS, Nishiyama T, Nagata Y, Kanemaki MT, Kawaguchi A, Ohkawa Y, Schermelleh L, Toyoda A, Xie L, Kurokawa K, Ochiai H, Sasai M, Maeshima K. Nat Genet. 2026 Sep;58(9):2335-2349. doi: 10.1038/s41588-026-02736-2. Epub 2026 Sep 8. 10.1038/s41588-026-02736-2 PubMed 42711428
Related items: