Skip to main content
Addgene
Open Menu

pEGFPC1-Cep89
(Plasmid #261521)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 261521 Standard format: Plasmid sent in bacteria as agar stab 1 $94
Add to Cart

Backbone

  • Vector backbone
    pEGFPC1
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Cep89
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Cep89 (a.k.a. 2610507L03Rik, Ccdc123)
  • Promoter CMV
  • Tag / Fusion Protein
    • GFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SalI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that the depositor's annotated reference file has differences compared to the Addgene-verified sequence. Compared to the depositor reference sequence, the Addgene sequence finds S190del and V418I in Cep89.

The depositor confirms that these discrepancies are not expected to alter the plasmid's function, and that they reflect the construct used in their study. Please refer to the the Addgene sequence for the most accurate result.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEGFPC1-Cep89 was a gift from Aimin Liu (Addgene plasmid # 261521 ; http://n2t.net/addgene:261521 ; RRID:Addgene_261521)

  • For your References section:

    C2cd3 is critical for centriolar distal appendage assembly and ciliary vesicle docking in mammals. Ye X, Zeng H, Ning G, Reiter JF, Liu A. Proc Natl Acad Sci U S A. 2014 Feb 11;111(6):2164-9. doi: 10.1073/pnas.1318737111. Epub 2014 Jan 27. 10.1073/pnas.1318737111 PubMed 24469809
Related items: