pEGFPC1-Vangl2
(Plasmid
#261524)
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PurposeC-terminal GFP-tagged mouse Vangl2
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Depositing Lab
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Depositing OrganizationPennsylvania State University
Located in the United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 261524 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepEGFPN1
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameVangl2
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SpeciesM. musculus (mouse)
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Entrez GeneVangl2 (a.k.a. C530001F03Rik, Lootl, Lp, Lpp1, Ltap, Vang1l2, loop-tail, ska17, ska<m17Jus>, stbm, strabismus)
- Promoter CMV
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Tag
/ Fusion Protein
- GFP (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NheI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEGFPC1-Vangl2 was a gift from Aimin Liu (Addgene plasmid # 261524 ; http://n2t.net/addgene:261524 ; RRID:Addgene_261524) -
For your References section:
TMEM132A regulates mouse hindgut morphogenesis and caudal development. Zeng H, Liu A. Development. 2023 Jul 15;150(14):dev201630. doi: 10.1242/dev.201630. Epub 2023 Jul 13. 10.1242/dev.201630 PubMed 37390294