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pcDNA3.2-T132a_3xFLAG
(Plasmid #261525)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 261525 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pcDNA3.2
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    EPI300
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Tmem132a
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Tmem132a (a.k.a. 6720481D13Rik, Hspa5bp1, Orai1)
  • Promoter CMV
  • Tag / Fusion Protein
    • 3xFLAG (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site HindIII (not destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.2-T132a_3xFLAG was a gift from Aimin Liu (Addgene plasmid # 261525 ; http://n2t.net/addgene:261525 ; RRID:Addgene_261525)

  • For your References section:

    TMEM132A regulates mouse hindgut morphogenesis and caudal development. Zeng H, Liu A. Development. 2023 Jul 15;150(14):dev201630. doi: 10.1242/dev.201630. Epub 2023 Jul 13. 10.1242/dev.201630 PubMed 37390294
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