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pAV-CamKIIa-ChrimsonR-mRuby2-ST
(Plasmid #261576)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 261576 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pAV
  • Backbone size w/o insert (bp) 5180
  • Total vector size (bp) 7183
  • Vector type
    Mammalian Expression, AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    ChrimsonR-mRuby2-ST
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2001
  • Promoter CamKIIa
  • Tags / Fusion Proteins
    • mRuby2 (C terminal on insert)
    • soma targeting motif (ST) (C terminal on insert)

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer GCCACAGTGCCCTGCTCA
  • 3′ sequencing primer CGAGGTCGACGGTATCG
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Vigene

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Subcloned from:

pAAV-CaMKIIa-eGFP-Cre
(Plasmid #120219)

pAAV-hSyn-DIO-ChrimsonR-mRuby2-ST
(Plasmid and Viral Vector Prep for #105448)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAV-CamKIIa-ChrimsonR-mRuby2-ST was a gift from Mark Histed (Addgene plasmid # 261576 ; http://n2t.net/addgene:261576 ; RRID:Addgene_261576)

  • For your References section:

    Excitation creates a distributed pattern of cortical suppression due to varied recurrent input. O'Rawe JF, Zhou Z, Li AJ, LaFosse PK, Goldbach HC, Histed MH. Neuron. 2023 Dec 20;111(24):4086-4101.e5. doi: 10.1016/j.neuron.2023.09.010. Epub 2023 Oct 20. 10.1016/j.neuron.2023.09.010 PubMed 37865083
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