pWN_U6-MCS-miniGag
(Plasmid
#261578)
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PurposeminiEDV production plasmid. Expresses the miniGag (CAG promoter) and sgRNA (human U6 promoter). Includes BsmBI sites for cloning in new sgRNA.
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Depositing Lab
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Depositing OrganizationUniversity of California, Berkeley
Located in United States of America -
Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 261578 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepJRH-1180 U6-reci Gag-pol v2
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Backbone manufacturerJennifer Doudna, Addgene plasmid #201915
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameminiGag
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pWN_U6-MCS-miniGag was a gift from Jennifer Doudna (Addgene plasmid # 261578 ; http://n2t.net/addgene:261578 ; RRID:Addgene_261578) -
For your References section:
Mechanism-guided engineering of a minimal biological particle for genome editing. Ngo W, Peukes J, Baldwin A, Xue ZW, Hwang S, Stickels RR, Lin Z, Satpathy AT, Wells JA, Schekman R, Nogales E, Doudna JA. Proc Natl Acad Sci U S A. 2025 Jan 7;122(1):e2413519121. doi: 10.1073/pnas.2413519121. Epub 2024 Dec 30. 10.1073/pnas.2413519121 PubMed 39793042
