pAAV.TBG.(cyto).iG6PSnFR-D.mIRFP
(Plasmid
#261599)
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PurposeCytosolic expression of iG6PSnFR-D with mIRFP670nano3 for normalization
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Depositing Lab
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Depositing OrganizationHHMI, Janelia Research Campus
Located in United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 261599 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepAAV
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Vector typeAAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Growth instructionsCan also be grown in Mach1 cells.
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameiG6PSnFR-D
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SpeciesM. musculus (mouse), Synthetic
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Tag
/ Fusion Protein
- mIRFP670nano3 (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unspecified (unknown if destroyed)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAV.TBG.(cyto).iG6PSnFR-D.mIRFP was a gift from Tim Brown (Addgene plasmid # 261599 ; http://n2t.net/addgene:261599 ; RRID:Addgene_261599) -
For your References section:
iG6PSnFR: A genetically encoded fluorescent sensor for observing glucose-6-phosphate dynamics in living preparations. Marvin JS, Tsives A, Huang SM, Koshenov Z, Adhikari R, Wolfe AD, Gonzalez IJ, Feliciano D, Ryan TA, Merrins MJ, Colon-Ramos DA, Brown TA. bioRxiv [Preprint]. 2026 Jul 23:2026.07.22.740099. doi: 10.64898/2026.07.22.740099. 10.64898/2026.07.22.740099 PubMed 42539160