pAd-ChR2-P2A-EGFP
(Plasmid
#261664)
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PurposeAdenoviral vector expressing ChR2(H134R)-P2A-EGFP under the CMV promoter for optogenetic activation and fluorescent labeling of transduced cells.
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Depositing Lab
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Depositing OrganizationBoston Children's Hospital
Located in United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 261664 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backboneAdenovirus
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Vector typeMammalian Expression, Adenoviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)EPI300
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameChR2(H134R)-P2A-EGFP
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Alt namechannelrhodopsin-2 H134R mutant
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SpeciesChlamydomonas reinhardtii
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Insert Size (bp)1711
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MutationH134R
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GenBank IDEU714030.1
- Promoter CMV
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer TAATACGACTCACTATAGG
- (Common Sequencing Primers)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAd-ChR2-P2A-EGFP was a gift from William Pu (Addgene plasmid # 261664 ; http://n2t.net/addgene:261664 ; RRID:Addgene_261664) -
For your References section:
Efficient and reproducible generation of human iPSC-derived cardiomyocytes and cardiac organoids in stirred suspension systems. Prondzynski M, Berkson P, Trembley MA, Tharani Y, Shani K, Bortolin RH, Sweat ME, Mayourian J, Yucel D, Cordoves AM, Gabbin B, Hou C, Anyanwu NJ, Nawar F, Cotton J, Milosh J, Walker D, Zhang Y, Lu F, Liu X, Parker KK, Bezzerides VJ, Pu WT. Nat Commun. 2024 Jul 15;15(1):5929. doi: 10.1038/s41467-024-50224-0. 10.1038/s41467-024-50224-0 PubMed 39009604