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pVGI-1084-C77G
(Plasmid #261732)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 261732 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone size w/o insert (bp) 4690
  • Total vector size (bp) 24056
  • Modifications to backbone
    An AscI and an FseI were introduced into the multiple cloning site. The CD45 minigene with the C77G mutation is inserted between the Asc1 and FseI sites. Two LoxP sequences were introduced, one between the Asc1 site and the start of the minigene and another at the end of the minigene in front of the FseI site.
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Murine CD45.1 Minigene
  • Alt name
    PTPRC-a Minigene
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    17513
  • Mutation
    C77G mutation (cysteine to glycine at amino acid position 77) in exon 4 that disrupts normal splicing of this exon. A single C to G nucleotide exchange in exon 4 was introduced into pVGI-1084 to generate C77G CD45.1 expression.
  • GenBank ID
    NC_000067.7
  • Entrez Gene
    Ptprc (a.k.a. B220, CD45R, Cd45, L-CA, Ly-5, Lyt-4, T200, loc)
  • Promoter human LFA

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Asc1 introduced into the multiple cloning site. (not destroyed)
  • 3′ cloning site Fse1 introduced into the multiple cloning site (not destroyed)
  • 5′ sequencing primer ccgacaattgcatgaag
  • 3′ sequencing primer ggcacagtcgaggctg
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

pVGI-1084-C77G differs from pVGI-1084 (Addgene #261400) by one nucleotide change, a C to a G at position 5,886

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pVGI-1084-C77G was a gift from William Raschke (Addgene plasmid # 261732 ; http://n2t.net/addgene:261732 ; RRID:Addgene_261732)
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