pcDNA3.1(+)-Flag-TDP43 S332N
(Plasmid
#261743)
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PurposeExpresses N-terminal Flag-tagged TDP-43 S332N in mammalian cells
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Depositing Lab
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Depositing OrganizationMonash University, Sunway Campus
Located in Malaysia -
Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 261743 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepcDNA3.1(+)
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTAR DNA-binding protein 43
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SpeciesH. sapiens (human)
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Mutationchanged Serine (S) 332 to Asparagine (N)
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Entrez GeneTARDBP (a.k.a. ALS10, TDP-43)
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Tag
/ Fusion Protein
- Flag tag (N terminal on insert)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3.1(+)-Flag-TDP43 S332N was a gift from Chen Seng Ng (Addgene plasmid # 261743 ; http://n2t.net/addgene:261743 ; RRID:Addgene_261743) -
For your References section:
SKALE: An Interpretable Multiscale Machine Learning Model for Decoding Phase-Specific Protein Aggregation in Neurodegenerative Proteinopathies. Loo WXW, Sio JS, Yap K, Loo YS, Lim HX, Zhang S, Liu H, Ng CS. Aggregate, 2026 10.1002/agt2.70280