pAL11
(Plasmid
#261764)
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Purpose(Empty Backbone) Empty backbone to clone sgRNA units with LacZ gene for blue white screening
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Depositing Lab
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Depositing OrganizationUniversity of British Columbia
Located in Canada -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 261764 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepU6-pegRNA-GG-acceptor
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Backbone manufacturerDavid Liu (Addgene #132777)
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Vector typeMammalian Expression, CRISPR
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
pU6-pegRNA-GG-acceptor modified to have the lacZ cloning site for sgRNA unit assembly.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAL11 was a gift from Carl de Boer (Addgene plasmid # 261764 ; http://n2t.net/addgene:261764 ; RRID:Addgene_261764)