Skip to main content
Addgene
Open Menu

HEK3-sgRNA
(Plasmid #261765)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 261765 Standard format: Plasmid sent in bacteria as agar stab 1 $94
Add to Cart

Backbone

  • Vector backbone
    pAL11
  • Vector type
    Mammalian Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HEK3-sgRNA
  • gRNA/shRNA sequence
    ggcccagactgagcacgtga
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    20
  • Promoter U6

Cloning Information

  • Cloning method Golden Gate

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

HEK3 sgRNA expression unit (U6 promoter-spacer-scaffold/terminator) cloned into pALB11 via Golden Gate assembly.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HEK3-sgRNA was a gift from Carl de Boer (Addgene plasmid # 261765 ; http://n2t.net/addgene:261765 ; RRID:Addgene_261765)
Related items: