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PDCD1-sgRNA
(Plasmid #261767)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 261767 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pAL11
  • Vector type
    Mammalian Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PDCD1-sgRNA
  • gRNA/shRNA sequence
    cacctacctaagaaccatcc
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    20
  • Entrez Gene
    PDCD1 (a.k.a. AIMTBS, CD279, PD-1, PD1, SLEB2, hPD-1, hPD-l, hSLE1)
  • Promoter U6

Cloning Information

  • Cloning method Golden Gate

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

PDCD1 sgRNA expression unit (U6 promoter-spacer-scaffold/terminator) cloned into pALB11 via Golden Gate assembly.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PDCD1-sgRNA was a gift from Carl de Boer (Addgene plasmid # 261767 ; http://n2t.net/addgene:261767 ; RRID:Addgene_261767)
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