pLJM1_Mem-mNG
(Plasmid
#261787)
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Purposetargets mNeonGreen to the plasma membrane via the membrane localization motif (MGCVCSSNPE) from LCK
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Depositing Lab
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Depositing OrganizationSouth Dakota State University
Located in the United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 261787 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepLJM1
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Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemem-mNG
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SpeciesH. sapiens (human)
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Insert Size (bp)781
- Promoter CMV
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Tag
/ Fusion Protein
- mNeon Green (C terminal on insert)
Cloning Information
- Cloning method Other
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLJM1_Mem-mNG was a gift from Natalie Thiex (Addgene plasmid # 261787 ; http://n2t.net/addgene:261787 ; RRID:Addgene_261787) -
For your References section:
The structural dynamics of macropinosome formation and PI3-kinase-mediated sealing revealed by lattice light sheet microscopy. Quinn SE, Huang L, Kerkvliet JG, Swanson JA, Smith S, Hoppe AD, Anderson RB, Thiex NW, Scott BL. Nat Commun. 2021 Aug 10;12(1):4838. doi: 10.1038/s41467-021-25187-1. 10.1038/s41467-021-25187-1 PubMed 34376698