hSyn-PSD95-Δ1.2-GFP
(Plasmid
#261816)
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PurposeExpresses PDZ domain deleted PSD95-GFP
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Depositing Lab
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Depositing OrganizationMax Planck Institute for Biological Intelligence
Located in Germany -
Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 261816 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepAAV-EF1 alpha-F-FLEX-Kir2.1-T2-tdTomato
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Backbone manufacturerMassimo Scanziani (Addgene #60661)
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Vector typeAAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePSD95
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SpeciesM. musculus (mouse)
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Insert Size (bp)2172
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MutationPDZ 1 and 2 deleted (aa 65-312)
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Entrez GeneDlg4 (a.k.a. Dlgh4, PSD-95, PSD95, SAP90, SAP90A)
- Promoter hSyn
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Tag
/ Fusion Protein
- EGFP (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
- 5′ sequencing primer n/a
- (Common Sequencing Primers)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
hSyn-PSD95-Δ1.2-GFP was a gift from Tobias Bonhoeffer (Addgene plasmid # 261816 ; http://n2t.net/addgene:261816 ; RRID:Addgene_261816) -
For your References section:
Deleting learning-induced dendritic spines disrupts the memory they encode. Joag H, Opazo P, Hagihara KM, Lüthi A, Bonhoeffer T. bioRxiv 2026.08.18.745461 10.64898/2026.08.18.745461