pAAV-TRE3G-dTAG-s-Drebrin-mRuby2-WPRE
(Plasmid
#261819)
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PurposeDoxycycline dependent dTAG-s-Drebrin-mRuby2 expression
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Depositing Lab
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Depositing OrganizationMax Planck Institute for Biological Intelligence
Located in Germany -
Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 261819 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backboneAAV2-ITR
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Backbone manufacturerVectorBuilder VB200224
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Vector typeAAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameDrebrin A
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SpeciesMammalian
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Insert Size (bp)2151
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Mutationincludes aa 1-366 only
- Promoter TRE3G
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Tags
/ Fusion Proteins
- dTAG (N terminal on insert)
- mRuby2 (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Plasmid contains a 22 base pair deletion in the 5' ITR. Depositor confirms no effect on plasmid's functionality.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAV-TRE3G-dTAG-s-Drebrin-mRuby2-WPRE was a gift from Tobias Bonhoeffer (Addgene plasmid # 261819 ; http://n2t.net/addgene:261819 ; RRID:Addgene_261819) -
For your References section:
Deleting learning-induced dendritic spines disrupts the memory they encode. Joag H, Opazo P, Hagihara KM, Lüthi A, Bonhoeffer T. bioRxiv 2026.08.18.745461 10.64898/2026.08.18.745461