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23SrRNA.RAV12(wt).2508-2580.GAAAC.duplexH90
(Plasmid #26192)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 26192 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    modified pUC19
  • Backbone size w/o insert (bp) 2876
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    23S ribosomal RNA fragment (modified) with duplex H90
  • Alt name
    23S rRNA
  • Insert Size (bp)
    70
  • Mutation
    fragment 2508-2580 of E. coli rRNA, with nucleotides 2530-2534 replaced with GAAAC and an rA 3' to nucleotide 2580; nucleotides 2572-2576 replaced with single G to give duplex H90

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site NgoMIV (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer Lac promoter
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    23SrRNA.RAV12(wt).2508-2580.GAAAC.duplexH90 was a gift from David McKay (Addgene plasmid # 26192 ; http://n2t.net/addgene:26192 ; RRID:Addgene_26192)
  • For your References section:

    Structure of the RNA Binding Domain of a DEAD-Box Helicase Bound to Its Ribosomal RNA Target Reveals a Novel Mode of Recognition by an RNA Recognition Motif. Hardin JW, Hu YX, McKay DB. J Mol Biol. 2010 Jul 29. ():. 10.1016/j.jmb.2010.07.040 PubMed 20673833