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pMG-101
(Plasmid #262056)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 262056 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pJCT039
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    aprE
  • Species
    B. subtilis
  • Mutation
    pMG-86 empty vector for aprE 5UTR MPRA library
  • Promoter pTet

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Gene/Insert 2

  • Gene/Insert name
    tetR
  • Species
    Synthetic

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2025.11.27.690947 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMG-101 was a gift from Gene-Wei Li (Addgene plasmid # 262056 ; http://n2t.net/addgene:262056 ; RRID:Addgene_262056)

  • For your References section:

    Long-range mRNA folding shapes expression and sequence of bacterial genes. Gill MS, Kim IA, Xue JR, Thappeta Y, Taggart JC, Li GW. Mol Cell. 2026 Sep 17;86(18):3789-3802.e11. doi: 10.1016/j.molcel.2026.08.014. Epub 2026 Sep 8. 10.1016/j.molcel.2026.08.014 PubMed 42710496
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